LOCUS       sequence-92045-e         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric (Blue to Green) fluorescent indicator for calcium 
            signaling in the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-13"; volume: "5:4153. doi"; pages: " 
            10.1038/ncomms5153"
COMMENT     Sequence Label: pCIS GEM-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     join(22..66,149..160)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(22..67,150..160)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             197..253
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     466..487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtatcgata agcttgccac catgggatgg agctgtatca tcctcttctt ggtagcaaca
       61 gctacaggta aggggttaac agtagcaggc ttgaggtctg gacatatata tgggtgacaa
      121 tgacatccac tttgcctttc tctccacagg cgcgcactcc caggtccaac tgcagggatc
      181 catggtcgac tcttcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg
      241 tcggctgagc tcaccagaga acgtgtatat aaaggccgac gagcagaaga acggcatcaa
      301 ggcgtacttc aagatccgcc acaacatcga gggcggcggc gtgcagctcg cctaccacta
      361 ccagcagatc acccccatag gcgacggccc cgtgctgctg cccgacaacc actacctgag
      421 cgtgcagtcc atactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      481 gttcgtgacc gccgccggga tcactctc
//
