LOCUS       sequence-92193-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 1.3 kb promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh1.3K-Luc
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    6..87
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     31..50
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(512..532)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
       61 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      121 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
      181 gctgattatg atctctagtc aaggcactat acatcaaata ttccttatta acccctttac
      241 aaattaaaaa gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat
      301 gcctgtgtgg agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa
      361 aggttacaga atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
      421 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
      481 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
      541 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagc
//
