LOCUS       sequence-92217-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to
            drive short RNAs, pgkneo, cassette flanked by loxP sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pRUSH
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    49..273
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    325..549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(786..819)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acgagctgta caagtaaagc ggccctagag ctcgctgatc agcctcgact gtgccttcta
       61 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      121 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      181 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      241 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
      301 gctcgactcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      361 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      421 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      481 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
      541 gctctatggc ttctgaggcg gaaagaacca gctggggctc gatcctctag agatccgacg
      601 cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact
      661 cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg
      721 ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga taggcttgga
      781 tttctataac ttcgtatagc atacattata cgaagttat
//
