LOCUS       sequence-92221-e         465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without REC2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX543 SpCas9(del175-307)
FEATURES             Location/Qualifiers
     source          1..465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..120
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             127..147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat
       61 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag
      121 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
      181 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      241 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      301 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      361 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      421 atcttcagca acgagatggc caaggtggac gacagcttct tccac
//
