LOCUS       sequence-92226-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TagRFP-T and presynaptic mGRASP component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4RFP-5premGRASP
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             235..282
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 ccgatctgcg acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac
       61 aagtaatgat taattaacgt cctttcaacg atccaagtcc atgaaaaaaa ctaacacccc
      121 tcccgtacga aagccgccac catgccacct tctactagtc ttttgttgct tgctgcactt
      181 ctccccttcg ctcttcccgc ttcagattgg aagacaggag aagtcaccgc ttcccgcgac
      241 cacatggtgc tgcacgaata tgtgaacgct gctggcatca caggcggtgg cggatctggc
      301 ggcggtggta gcgtcgactt tcagaaagct agctctattg tttataagaa ggagggggaa
      361 caggtggagt tcagtttccc attggccttc acagtcgaga agctcacagg atcaggagag
      421 ttgtggtggc aggccgaaag agcctcctcc agcaagagtt ggatcacttt tgatctgaaa
      481 aacaaagaag tgagcgtgaa gagagtcaca caggacccta agctgcagat gggcaagaag
      541 ttgcctttgc acttgacact cccccaagcc ctgccccagt atgctggctc cgggaacctc
      601 accctcgcct tggaggctaa gaccggaaag ttgcatcagg aggtcaacct cgtggtgatg
      661 agggcaacac agctgcagaa gaacctgacc tgcgaagtgt ggggtcccac ctcacctaaa
      721 ctgatgctgt ctctgaagct ggagaataag gaggctaaag tcagtaaaag agagaaggct
      781 gtgtgggtcc ttaacccgga ggcagggatg tggcagtgtc ttcttagcga ctctggacag
      841 gtgctgctgg agagcaacat caaggtgctt cctacttggt caacaccagt acagccgatg
      901 gc
//
