LOCUS       sequence-92231-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GCaMP6s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4GCaMP6s
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             169..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             190..222
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(226..234,235..249)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      256..265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             277..333
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     546..567
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(673..694)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aactccaacc cttgggagca atataacaaa aaacatgtta tggtgccatt aaaccgctgc
       61 atttcatcaa agtcaagttg attaccttta cattttgatc ctcttggatg tgaaaaaaac
      121 tattaacatc cctcaaaaga ccccgggaaa gccgccacca tgggttctca tcatcatcat
      181 catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat
      241 gacgataagg atctcgccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt
      301 cacgcagtca gagctatagg tcggctgagc tcactcgaga acgtctatat caaggccgac
      361 aagcagaaga acggcatcaa ggcgaactnc cacatccgcc acaacatcga ggacggcggc
      421 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      481 cccgacaacc actacctgag cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc
      541 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      601 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
      661 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      721 ggcgagggtg agggcgatgc
//
