LOCUS       sequence-92250-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEL-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..809)
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgctt atcgtctggc attgtcaggc aatccagaat gagcaacagc atgctcatac
      181 agcttaacct tgttgtaatc tttgtcatgg cttaaaatct caatgcagtg gtccacaaag
      241 tgggcgcctg gtaacttgac acccttctcc ttagctttgt aagtagttct gaagtcacat
      301 cggtaatggg catttccttc aagcaacaaa gccatctcta catcacccgt cagcactcca
      361 tcacgcacat acattttctc agtggagggc tcccatttca gcgtcttctt ctgcataact
      421 ggaccattgg cgggaaagtt ggtaccataa aatcgaactt tattatagaa agtgtcccct
      481 tccattgtta tgtcgtttct ggcgttgcaa atgcccccgt cttcgaaagt caagcttcgt
      541 tcccacgaat accccttagg aaacgactgc ttaaaatagt cttgtatgtt gtctggatat
      601 ttggcgaata ccctgttgcc gtacaggaat gcagtggtca ggatatcaaa ggcaaaaggc
      661 agaggtccgc cctctttgac ttcaagatcc atactctgtt ttccctcaaa aggcttgcct
      721 gtaccatctc cgtcgatcac aaagtggtgc ccgtttacgt tgccttccat acggagtttg
      781 atcttcatgt ctggcttaat cgcactcatt ggatcccgtt cttcctttga gatgctttcg
      841 a
//
