LOCUS       sequence-92382-e        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA SF-HERC2 F4
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..178
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             188..211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac gcgtccacca tggactacaa
      121 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg
      181 gtcggcctgg agccacccgc agttcgaaaa aggatccact agtccagtgt ggtggaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtgatgga ttgcatgatc tcaatgtgca
      301 gtgtgactgg cagcagaaag ggggcaccta ctgggttagg tacattcatg tggaacttat
      361 aggctatcct ccaccaagtt cttcttctca catcaagatt ggtgataaag tgcgggtcaa
      421 agcctctgtc accacaccaa aatacaaatg gggatctgtg actcatcaga gtgtgggggt
      481 tgtgaaagct ttcagtgcca atggaaaaga tatcattgtc gactttcccc agcagtctca
      541 ctggactggg ttgctatcag aaatggagtt ggtacccagt attcatcctg gggttacgtg
      601 tgatggatgt cagatgtttc ctatcaatgg atccagattc aaatgcagaa actgtgatga
      661 ctttgatttt tgtgaaacgt gtttcaagac caaaaaacac aataccaggc atacatttgg
      721 cagaataaat gaaccaggtc agtctgcggt attttgtggc cgttctggaa aacagctgaa
      781 gcgttgccac agcagccagc caggcatgct gctggacagc tggtcccgca tggtgaagag
      841 cctgaatgtg tcgtcctccg tgaaccaggc atcccgtctc attgacggca gcgagccctg
      901 ctggcagtca tcggggtcgc aaggaaagca ctggattcgt ttggagattt tcccagatgt
      961 tcttgttcat agattaaaaa tgatcgtaga tcctgctgac agtagctaca tgccgtccct
     1021 ggttgtagtg tcaggtggaa attccctgaa taaccttatt gaactaaaga caatcaatat
     1081 taacccttct gacaccacag tgccccttct gaatgactgc acagag
//
