LOCUS       sequence-92440-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-Spec(x2)-DocI
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             173..196
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             203..220
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atgggtaccg actctctgga attcatcgct tctaaactgg ctctggaagt
      181 tctgttccag ggtccgctgc agcaccacca ccaccatcac ccatggacta gtgctagccg
      241 tgctaaactg aacgaatctc accgtctgca ccagttcttc cgtgacatgg acgacgaaga
      301 atcttggatc aaagaaaaaa aactgctggt ttcttctgaa gactacggtc gtgacctgac
      361 cggtgttcag aacctgcgta aaaaacacaa acgtctggaa gctgaactgg ctgctcacga
      421 accggctatc cagggtgttc tggacaccgg taaaaaactg tctgacgaca acaccatcgg
      481 taaagaagaa atccagcagc gtctggctca gttcgttgac cactggaaag aactgaaaca
      541 gctggctgct gctcgtggtc agcgtctgga agaatctctg gaatacggta gcggtagcgg
      601 ttcacgtgct aaactgaacg aatctcaccg tctgcaccag ttcttccgtg acatggacga
      661 cgaagaatct tggatcaaag aaaaaaaact gctggtttct tctgaagact acggtcgtga
      721 cctgaccggt gttcagaacc tgcgtaaaaa acacaaacgt ctgg
//
