LOCUS       sequence-92469-e         437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  is a lentiviral vector coding the shRNA si1 (sigfp) and the gene of 
            resistance to puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 437)
  TITLE     biG-biS plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psp-161-si1(sigfp)
FEATURES             Location/Qualifiers
     source          1..437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        193..407
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     328..347
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
       61 aacggatctc gacggtatcg gatcccaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc
      181 aggctggaat tcgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc
      241 actaggcggg aacacccagc gcgcgtgcgc cctggcagga agatggctgt gagggacagg
      301 ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt
      361 gaaatgtctt tggatttggg aatcttataa gttctgtatg agaccacaga tccccgaacg
      421 gcatcaaggt gaacttc
//
