LOCUS       sequence-92528-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of bovine pancreatic ribonuclease A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT
  TITLE     Engineering ribonuclease A: production, purification and 
            characterization of wild-type enzyme and mutants at Gln11.
  JOURNAL   Protein Eng. 1995 Mar;8(3):261-73.
  PUBMED    7479688
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng."; date: "1995-03"; volume: "8(3)"; pages: "261-73"
COMMENT     Sequence Label: pET22b RNase A
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     33..98
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             99..143
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             503..520
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(573..591)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      587..634
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgaaata cctgctgccg accgctgctg
       61 ctggtctgct gctcctcgct gcccagccgg cgatggccaa ggaaactgca gcagccaagt
      121 ttgagcggca gcacatggac tccagcactt ccgctgccag cagctccaac tactgtaacc
      181 agatgatgaa gagccggaac ctgaccaaag atcgatgcaa gccagtgaac acctttgtgc
      241 acgagtccct ggctgatgtc caggccgtgt gctcccagaa aaatgttgcc tgcaagaatg
      301 ggcagaccaa ttgctaccag agctactcca ccatgagcat caccgactgc cgtgagaccg
      361 gcagctccaa gtaccccaac tgtgcctaca agaccaccca ggcgaataaa cacatcattg
      421 tggcttgtga gggaaacccg tacgtgccag tccactttga tgcttcagtg tagtagtcga
      481 caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      541 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      601 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      661 ggcgaatggg acgcgccctg tagcggcg
//
