LOCUS       sequence-92530-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human PITP alpha N90F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Tilley SJ, Skippen A, Murray-Rust J, Swigart PM, Stewart A, Morgan 
            CP, Cockcroft S, McDonald NQ
  TITLE     Structure-function analysis of human [corrected] 
            phosphatidylinositol transfer protein alpha bound to 
            phosphatidylinositol.
  JOURNAL   Structure. 2004 Feb;12(2):317-26.
  PUBMED    14962392
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2004-02"; volume: "12(2)"; pages: "317-26"
COMMENT     Sequence Label: pET14b-PITPalpha N90F
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(844..861)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(871..888)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(960..979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(961..979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatccta gtcatctgct gtcattcctt tcactgggtc
       61 cttttgtctc atttcatcca gctgtctctt cgtctcttct tccatccttc gaatgtcgtc
      121 catggtcagg tcaacccact tatcgagcca acagaacagc tgcctgtgga agtttgtaaa
      181 cagacgcctc tcttgcttat ggatgaagtt ctccactttg ttctgcaggc cccaccactt
      241 gaacttgacg gtcaccagtt tgtatgcaca catatatggg cagtccttct ggtttaccag
      301 ctcttgcttc caattggggc ccaagggtcc tcggcctgtt ttgatagatt taaattttgc
      361 tgggtcttcc tctgccttgt aatccttgct gagcacttgg cttcgatctg caatgtctat
      421 atatacggct tccacgtgtt tccacgcctc aggctccagc ttatgcacat tctcctgcgt
      481 gccaagatct ggtttgtgcc aggtttcaat tttaatcaga aagtcttctt tcatgtactc
      541 atttgtaata acggttctgc agtaggggta agcaaaccag gctttctcgt gtatattcag
      601 ggctccctct ggggccagca ttcgaacaaa cgtgggtact ttgctctgca ggtggtagat
      661 cttgtgtgtg tactggcctt tctcaccgtc cttctcgtag ggctcattca ccaggacctc
      721 cacgccttcg ccaccacccg tttcattttt actggcctca gccacagaat acagctgccc
      781 cacttgatac tcatctacag acacaggcag gattactcga tactccttga gcagcaccat
      841 atggctgccg cgcggcacca ggccgctgct gtgatgatga tgatgatggc tgctgcccat
      901 ggtatatctc cttcttaaag taaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      961 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccgga
//
