LOCUS       sequence-92541-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation module. The plasmid consists of a mutant T7 RNA 
            polymerase (T7 RNAP) activating its own expression via a T7 promoter
            carrying alac operator. Carbenicillin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Payne S, Li B, Cao Y, Schaeffer D, Ryser MD, You L
  TITLE     Temporal control of self-organized pattern formation without 
            morphogen gradients in bacteria.
  JOURNAL   Mol Syst Biol. 2013 Oct 8;9:697. doi: 10.1038/msb.2013.55.
  PUBMED    24104480
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-8"; volume: "9:697. doi"; pages: " 
            10.1038/msb.2013.55"
COMMENT     Sequence Label: pET15bLCFPT7
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             30..41
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ataagagctc cattagcatc gctagcatta aagaggagaa agctaccgca tgaacacgat
       61 taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct
      121 ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta
      181 cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctgtgaggtt
      241 gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc
      301 atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag
      361 ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct
      421 tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc
      481 attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa
      541 aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa
      601 gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg
      661 cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc
      721 ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc
      781 gaactcgcac ctgaatacgc tgaggctatc gcacc
//
