LOCUS       sequence-92557-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette to replace yeast promoters with an inducible promoter 
            containing 4 lexA boxes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-Pmin...
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..377
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cttgccttgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
       61 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
      121 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
      181 aaaaattacg gctcctcgct gcggacctgc gagcagggaa acgctcccct cacagacgcg
      241 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
      301 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
      361 tccgaacata aacaaccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      421 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      481 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      541 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
      601 ttccggaagt gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc
      661 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
      721 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
      781 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
      841 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
      901 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
      961 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     1021 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     1081 t
//
