LOCUS       sequence-92558-e        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette to replace yeast promoters with an inducible promoter 
            containing 4 lexA boxes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-Pmin...
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8..24
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(472..669)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
       61 ccactagtat agaagtatag taatttatgc tgcaaaggtc ctaatgtata aggaaagaag
      121 agaaaagaag aaaacaagag ttttatatac atacagagca catgctctag accctgggtg
      181 tatatacagt tatgctgtga gtatatacag cacctagacc ctgggtgtat atacagttat
      241 gctgtgagta tatacagcac ctagaccctg ggtgtatata cagttatgct gtgagtatat
      301 acagcaccta gaccctgggt gtatatacag ttatgctgtg agtatataca gcacctaggg
      361 agctcgaaaa gaaatatagt cttcaaggtt atattataca ggtttatata ttattgcggc
      421 cgcactagtt cgtttaaaca ctggatggcg gcgttagtat cgaatcgaca gcagtatagc
      481 gaccagcatt cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc
      541 tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag
      601 aacaactaca atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt
      661 attgtcagta ctgattattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
      721 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
      781 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
      841 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
      901 agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag
      961 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
     1021 tattgggaat ccccgaacat c
//
