LOCUS       sequence-9266-ex       10756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC247
FEATURES             Location/Qualifiers
     source          1..10756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..201
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(226..231,232..249,250..256)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             310..969
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1311..1616
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1657..1781)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1791..1838)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1914..1934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1956..1976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1998..2018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2040..2060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2103..2131)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2879..2899
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(3745..3792)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3867..3990
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4031..4336)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(4678..5337)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(5391..5397,5398..5415,5416..5421))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5447..5570)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6461..6472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8507..8611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8612..8680,8681..9472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8830..8849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9643..10231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10132..10151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10385..10402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10519..10540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10555..10560,10561..10578,10579..10585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10593..10609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10598..10620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10617..10633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10617..10633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
      121 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      181 acaacatatc cagtcatatt ggcggccgca ttaggcaccc caggctttac actttatgct
      241 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
      301 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      361 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      421 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      481 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
      541 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      601 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
      661 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
      721 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
      781 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
      841 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
      901 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
      961 ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     1021 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     1081 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     1141 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     1201 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     1261 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     1321 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     1381 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     1441 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     1501 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     1561 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     1621 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     1681 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     1741 tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatgctct gaagttccta
     1801 tactttctag agaataggaa cttcggaata ggaacttcaa gatccccggg tatgtttcga
     1861 atgatactaa cataacatag aacattttca ggagctcaga aaaaatgact gctccaaaga
     1921 agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     1981 cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa atgactgctc
     2041 caaagaagaa gcgtaaggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg
     2101 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     2161 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     2221 ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt
     2281 atttaaattt tcagccaaca cttgtcacta ctttctgtta tggtgttcaa tgcttctcga
     2341 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     2401 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt aagtttaaac
     2461 agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc aagtttgaag
     2521 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     2581 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     2641 aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact aactaatctg
     2701 atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc gttcaactag
     2761 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     2821 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     2881 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggacgaa ctatacaaaa
     2941 gcccacaagc tttggaattc gctagccggc catacaagta atccggatga tcgacgccaa
     3001 cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt
     3061 attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat
     3121 gagcggtttt ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac
     3181 aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg
     3241 gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa
     3301 cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg
     3361 aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct
     3421 tgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     3481 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     3541 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     3601 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     3661 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3721 taataatggt ttcttagacg ctctgaagtt cctatacttt ctagagaata ggaacttcgg
     3781 aataggaact tcaagatccc cgggtatgtt tcgaatgata ctaacataac atagaacatt
     3841 ttcaggagct cattacgcca agctatcaac tttgtataga aaagttgaac gagaaacgta
     3901 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     3961 ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg tgtataaggg
     4021 agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
     4081 ggtggctgag atcagccact tcttccccga taacggagac cggcacactg gccatatcgg
     4141 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacggggga
     4201 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
     4261 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
     4321 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc gttcatttca
     4381 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
     4441 agacgacggg cttcattctg catggttgtg cttaccgaac cggagatatt gacatcatat
     4501 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
     4561 catacctctt tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
     4621 atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc ctctagatta
     4681 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     4741 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     4801 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     4861 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     4921 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     4981 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     5041 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     5101 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
     5161 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     5221 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     5281 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     5341 agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga
     5401 gccggaagca taaagtgtaa agcctggggg tgcctaatgc ggccgccata gtgactggat
     5461 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     5521 gatatttata tcattttacg tttctcgttc aactttatta tacatagttg ataattcact
     5581 gggctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa
     5641 ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt
     5701 ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac atgagcggtt ttttttctct
     5761 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     5821 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     5881 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     5941 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa ctagtaaaaa
     6001 ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccct ttcgtctcgc
     6061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6181 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     6241 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta
     6301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6361 caacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat
     6421 atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt
     6481 ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca
     6541 aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca
     6601 tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt
     6661 cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc
     6721 cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc
     6781 ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga
     6841 agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga
     6901 gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac
     6961 caaaacgcat tcgtttttgg gattcacccc gcggttcgca acaggtttct ttttcaaatt
     7021 attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt
     7081 attttgatta catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca
     7141 tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag
     7201 ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt
     7261 ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaaca
     7321 atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa
     7381 accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact
     7441 tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa
     7501 tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc
     7561 actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg
     7621 ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga
     7681 cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc
     7741 gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa
     7801 aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat
     7861 gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt
     7921 ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc aactctctca
     7981 acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt
     8041 ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa
     8101 caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa
     8161 aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca
     8221 ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag
     8281 cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac
     8341 actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc
     8401 aactgaaaga ttttttcatt agagaatgtc taggcatgct ctagagggcc caaaaggcct
     8461 tttttttttg gcgccgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9121 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9781 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10021 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10261 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    10321 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10381 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    10441 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    10501 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    10561 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    10621 aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt actaactaac
    10681 tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac
    10741 gctaacaact tggaaa
//
