LOCUS       sequence-92750-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN R48W mutant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN R48W
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..65
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(85..108)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(127..156)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(697..716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(742..945)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(771..791)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggagaggcac tggggaaggg gtcacaggga tgccacccgg gatctgttca ggaaacagct
       61 atgaccgcgg ccggccgttt aaaccttatc gtcgtcatcc ttgtaatcca ggatatcatt
      121 tgctgccaga tcctcttctg agatgagttt ctgctcgagc ggccgcgtac gcgtgtcaca
      181 ctgctggaag tagaagtctg tgatgggggc attccagcca ccattctcgg gaagctgggt
      241 gagtctgaca ggctgatcgg cttcaggcac cgtgcacagg aaccagcccg ggtaggcagc
      301 cgactcgaag ctggaggtga gccccatgtc ccgccggtag aaggtgaagc tcttggattc
      361 cttggcacca agatagagct ccatgatgtt cactggctct agtgttagag tcggctcctg
      421 ccccacccca catgacaggc actggcttcc accctggaca cccaggatga cgggggacag
      481 gctggcatcc agccaccaat tggggaccac gctgatctct tcacctttaa tgaccttccc
      541 tgcatgcagc cctccagcta gaagctggtt attatgcaga taaagcacct tcaatgccga
      601 gtccttcatt cggaagcaca gcgccccact caggaccatg gcgatcgcgg cggcagatct
      661 cctcggtacc ggatccagtc gacgaattcc cggccgccct atagtgagtc gtattacaaa
      721 attctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
      781 gcccatttgc gtcaacgggg cggggttatt acgacatttt ggaaagtccc gttgattttg
      841 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
      901 aaaccgctat ccacgcccat tggtgtactg ccaaaaccgc atcaccatgg taatagcgat
      961 gactaatacg taaatgtact gccaagtagg aaagtcccgt aaggtcatgt actgggcata
//
