LOCUS       sequence-92757-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN R102W mutant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN R102W
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             589..618
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             637..660
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(680..696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agctcgttta gtgaccgtca gaattttgta atacgactca ctatagggcg gccgggaatt
       61 cgtcgactgg atccggtacc gaggagatct gccgccgcga tcgccatggt cctgagtggg
      121 gcgctgtgct tccgaatgaa ggactcggca ttgaaggtgc tttatctgca taataaccag
      181 cttctagctg gagggctgca tgcagggaag gtcattaaag gtgaagagat cagcgtggtc
      241 cccaatcggt ggctggatgc cagcctgtcc cccgtcatcc tgggtgtcca gggtggaagc
      301 cagtgcctgt catgtggggt ggggcaggag ccgactctaa cactagagcc agtgaacatc
      361 atggagctct atcttggtgc caaggaatcc aagagcttca ccttctactg gcgggacatg
      421 gggctcacct ccagcttcga gtcggctgcc tacccgggct ggttcctgtg cacggtgcct
      481 gaagccgatc agcctgtcag actcacccag cttcccgaga atggtggctg gaatgccccc
      541 atcacagact tctacttcca gcagtgtgac acgcgtacgc ggccgctcga gcagaaactc
      601 atctcagaag aggatctggc agcaaatgat atcctggatt acaaggatga cgacgataag
      661 gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt ggcatccctg
      721 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
      781 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
      841 ggggtggaag gggg
//
