LOCUS       sequence-9279-ex        4095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC379
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(78..125)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(175..203)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     951..971
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1535..1557)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1657..1676
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1754..1772)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1840..1944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1945..2013,2014..2805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2163..2182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2976..3564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3465..3484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3718..3735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3852..3873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3888..3893,3894..3911,3912..3918)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3926..3942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3931..3953
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3950..3966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3950..3966
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
      121 acttcaagat ccccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc
      181 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      241 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      301 ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
      361 tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca
      421 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      481 agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt
      541 actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc
      601 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      661 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      721 aatggaatca aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
      781 tttcagaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
      841 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      901 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      961 gagtttgtaa cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa
     1021 gctttggaat tcgctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg
     1081 aattttcaaa ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt
     1141 tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac atgagcggtt
     1201 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
     1261 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
     1321 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
     1381 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa
     1441 ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccct
     1501 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1621 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     1681 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1741 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1801 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3121 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3361 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3781 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3841 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3901 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3961 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
     4021 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
     4081 acttggaaat gaaat
//
