LOCUS       sequence-92802-e        1450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-Tre for expression of traptavidin (high stability mutant of 
            streptavidin) bearing a glutamic acid tag for ion-exchange 
            chromatography.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Traptavidin-E6
FEATURES             Location/Qualifiers
     source          1..1450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             477..494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(547..565)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      561..608
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      645..1098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(732..751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     942..963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1325..1337
                     /estimated_length=13
     gap             1435..1449
                     /estimated_length=15
ORIGIN
        1 ttccnnnnna nannnntttt gtttaacttt aagaaggaga tatacatatg gctgaagctg
       61 gtatcaccgg cacctggtac aaccagctgg gatccacctt catcgttacc gctggtgctg
      121 acggtgctct gaccggtacc tacgaatccg ctgttggtaa cgctgaaggc gattacgttc
      181 tgaccggtcg ttacgactcc gctccggcta ccgacggttc cggaaccgct ctgggttgga
      241 ccgttgcttg gaaaaacaac taccgtaacg ctcactccgc taccacctgg tctggccagt
      301 acgttggtgg tgctgaagct cgtatcaaca cccagtggtt gttgacctcc ggcaccaccg
      361 aagctaacgc gtggaaatcc accctggttg gtcacgacac cttcaccaaa gttaaaccgt
      421 ccgctgcttc cgaagaggaa gaggaagagt aataaaagct tgcggccgca ctcgagcacc
      481 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      541 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      601 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      841 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      901 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      961 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataanggn ntttgccgat
     1021 ttcggcctat tggttaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1081 tattaacgct tacaatttag nggcactttt cggggaaatg tgcgcgaacc ctatttgttt
     1141 atttttctaa atacattcaa tatgtatccg ctcatganan anancctgat aatgcttcan
     1201 taatattgaa aaggaagagt atgagtattc acatttccgn gtcgcctnat tccntttttt
     1261 gngnnatttg cnncnnnttt nnnnnnccnn aaannctggt gaagtaaana tgctganatc
     1321 antgnnnnnn nnnnnnncnt cnantnnnnn nnancgnaan attcntgann nntnnncccn
     1381 nanannnncn nnnnngnnnn nnttnannnc tgcnnnnnng gnnngnntnt tntcnnnnnn
     1441 nnnnnnnnng
//
