LOCUS       sequence-92920-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for homology-directed repair to 
            replace genes or other genomic sequence with an attP docking site. 
            Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings 
            AM, O'Connor-Giles KM
  TITLE     Highly specific and efficient CRISPR/Cas9-catalyzed 
            homology-directed repair in Drosophila.
  JOURNAL   Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. 
            Epub 2014 Jan 29.
  PUBMED    24478335
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.113.160713"; journalName: "Genetics"; date: 
            "2014-04"; volume: "196"; issue: "4"; pages: "961-71"
COMMENT     Sequence Label: pDsRed-attP
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    136..169
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(531..549)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(581..601)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     799..818
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgggctatta tggggtgtcg cccttcgctg aagcaggtgg aattcttgca tgctagcggc
       61 cgcggacata tgcacacctg cgatcgtagt gccccaactg gggtaacctt tgagttctct
      121 cagttggggg cgtagataac ttcgtataat gtatgctata cgaagttatc gtacgggatc
      181 taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat
      241 ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
      301 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
      361 ctgaacaagc taaacaatcg gctcgaagcc ggtcgccacc atggcctcct ccgaggacgt
      421 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt
      481 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      541 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta
      601 cggctccaag gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt
      661 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      721 gacccaggac tcctccctcc aggacggctc cttcatctac aaggtgaagt tcatcggcgt
      781 gaacttcccc tccgacggcc ccgtaatgca gaagaagact atgggctggg aggcgtccac
      841 cgagcgcctg tacccccgcg acggcgtgct
//
