LOCUS       sequence-92921-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for homology-directed repair to 
            replace genes or other genomic sequence with an attP docking site. 
            Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings 
            AM, O'Connor-Giles KM
  TITLE     Highly specific and efficient CRISPR/Cas9-catalyzed 
            homology-directed repair in Drosophila.
  JOURNAL   Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. 
            Epub 2014 Jan 29.
  PUBMED    24478335
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.113.160713"; journalName: "Genetics"; date: 
            "2014-04"; volume: "196"; issue: "4"; pages: "961-71"
COMMENT     Sequence Label: pDsRed-attP
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     150..173
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    356..477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(393..412)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     447..466
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    484..517
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa
       61 ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca
      121 gctgcccggc tactactacg tggactccaa gctggacatc acctcccaca acgaggacta
      181 caccatcgtg gagcagtacg agcgcgccga gggccgccac cacctgttcc tgtaggggcc
      241 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
      301 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      361 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      421 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacc
      481 ggtataactt cgtataatgt atgctatacg aagttataga agagcactag taaagatctc
      541 catgcataag gcgcgcctag gccttctgca gctcgaggct cttccgtcaa tcgagttcaa
      601 gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt atagtttgta
      661 ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt
      721 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca
      781 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa
      841 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat
      901 atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc ctaaa
//
