LOCUS       sequence-92936-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector (pT7) for untagged expression as well as fusions 
            to His6-SUMO or His6 tags. Designed for single or multiple fragment 
            cloning by yeast homologous recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Holmberg MA, Gowda NK, Andreasson C
  TITLE     A versatile bacterial expression vector designed for single-step 
            cloning of multiple DNA fragments using homologous recombination.
  JOURNAL   Protein Expr Purif. 2014 Jun;98:38-45. doi: 
            10.1016/j.pep.2014.03.002. Epub 2014 Mar 12.
  PUBMED    24631626
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2014-06"; volume: "98:38-45. doi"; pages: " 
            10.1016/j.pep.2014.03.002. Epub 2014 Mar 12"
COMMENT     Sequence Label: pSUMO-YHRC
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(35..378)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gcggcctcga aacgtgagtc ttttccttac ccatggttgt ttatgttcgg atgtgatgtg
       61 agaactgtat cctagcaaga ttttaaaagg aagtatatga aagaagaacc tcagtggcaa
      121 atcctaacct tttatatttc tctacagggg cgcggcgtgg ggacaattca acgcgtctgt
      181 gaggggagcg tttccctgct cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc
      241 cgtgcggcca tcaaaatgta tggatgcaaa tgattataca tggggatgta tgggctaaat
      301 gtacgggcga cagtcacatc atgcccctga gctgcgcacg tcaagactgt caaggagggt
      361 attctgggcc tccatgtcgc tggccgggtg acccggcggg gacgaggcaa gcgatcgctt
      421 gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct
      481 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
      541 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
      601 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
      661 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
      721 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
      781 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
//
