LOCUS       sequence-93039-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4NXJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MAC045-C12
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(426..446)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(459..476)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             486..508
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    523..547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(547..566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(548..566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttcgtcatc ataggtgtat gttcatatat ttcgtttgaa ttttaatatt taatttttta
      121 taataagttt ctatatcttc agcaaattta taatatattg tacttgggtc attatatgta
      181 taacaattat taataattaa aagaacatct ttctcaaatt cttgaaaact tttatattta
      241 aaattctgaa gtttctgttt cattgttgta aaatccatag gttcttttat gacattcaaa
      301 taatcaggta ctaactgtac attcacagga tataaaaata ttcttttctt atcaaaagct
      361 attagcttat ttaataaatt tgttagtact tcattcttgc tctttaattc ttcaatctcg
      421 tcatagccct ggaaatacaa gttttctctg ccgctgctgt gatgatgatg atgatgcatg
      481 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      541 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
      601 ggacgcatcg
//
