LOCUS       sequence-93063-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            tagRFP via P2A cleavage site. EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.EFS.tRFP
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..754)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(755..811)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(827..874)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cttaacgcgt tcaattaagt ttgtgcccca
       61 gtttgctagg gaggtcgcag tatctggcca cagccacctc gtgctgctcg acgtaggtct
      121 ctttgtcggc ctccttgatt ctttccagtc tgtggtccac atagtagacg ccgggcatct
      181 tgaggttctt agcgggtttc ttggatctgt atgtggtctt gaagttgcag atcaggtggc
      241 ccccgcccac gagcttcagg gccatgtcgc ttctgccttc caggccgccg tcagcggggt
      301 acagcatctc ggtgttggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg
      361 atgggaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc tggaggctgg
      421 tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga
      481 agccctcagg gaaggactgc ttaaagaagt cggggatgcc ctgggtgtgg ttgatgaagg
      541 ttctgctgcc gtacatgaag ctggtagcca ggatgtcgaa ggcgaagggg agagggccgc
      601 cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg
      661 atgtgcactt gaagtggtgg ttgttcacgg tgccctccat atacagcttc atgtgcatgt
      721 tctccttaat cagctcttcg cccttagaca ccatggggcc ggggttctcc tccacgtcgc
      781 cggcctgctt cagcaggctg aagttggtgg cgccgctgcc gctagctttc tttttcttag
      841 cttgaccagc tttcttagta gcagcaggac gcttgtcgcc tcccagctga gacaggtcga
      901 tccgtgtctc gtacagg
//
