LOCUS       sequence-93067-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin 
            resistance, EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pLKO5d.EFS.SpCas9.P2A.BSD
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..455)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(456..512)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(528..575)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 caaattttgt aatccagagg ttgattgtcg acttaacgcg tttactactg cagctgcagg
       61 ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca
      121 tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac
      181 cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg
      241 ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca
      301 gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg
      361 gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg
      421 agggtggatt cttcttgaga caaaggcttg gccatggggc cggggttctc ctccacgtcg
      481 ccggcctgct tcagcaggct gaagttggtg gcgccgctgc cgctagcttt ctttttctta
      541 gcttgaccag ctttcttagt agcagcagga cgcttgtcgc ctcccagctg agacaggtcg
      601 atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct
      661 ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg
      721 gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgatgggc
      781 ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc ggccaggatc
      841 actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt
      901 tccacaaaca gctgtttctg ctcat
//
