LOCUS       sequence-93069-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin 
            resistance, EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pLKO5d.EFS.SpCas9.P2A.BSD
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(314..334)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(341..364)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      364..373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(393..604)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    681..798
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agaagctgtc gtccaccttg gccatctcgt tgctgaagat ctcttgcaga tagcagatcc
       61 ggttcttccg tctggtgtat cttcttctgg cggttctctt cagccgggtg gcctcggctg
      121 tttcgccgct gtcgaacagc agggctccga tcaggttctt cttgatgctg tgccggtcgg
      181 tgttgcccag caccttgaat ttcttgctgg gcaccttgta ctcgtcggtg atcacggccc
      241 agcccacaga gttggtgccg atgtccaggc cgatgctgta cttcttgtcg gctgctggga
      301 ctccgtggat accgaccttc cgcttcttct ttggggccat cttatcgtca tcgtctttgt
      361 aatccatggt ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga
      421 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
      481 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
      541 ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
      601 gcccactgac gggcaccgga gccagcggcc gcctcgagcc ggcgccaaag tggatctctg
      661 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
      721 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
      781 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctcaagat ctagaattgt
      841 ccctcggggt tgggaggtgg gtctgaa
//
