LOCUS       sequence-93097-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 588, Emission:
            635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, 
            Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, 
            Chudakov DM
  TITLE     Bright far-red fluorescent protein for whole-body imaging.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):741-6.
  PUBMED    17721542
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):741-6."
COMMENT     Sequence Label: mKate-pBAD
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..69
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             155..175
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actctctact gtttctccat acccgttttt ttgggctaga aataattttg tttaacttta
       61 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
      121 gactggtgga cagcaaatgg gtcgggatct gtacgagaac ctgtacttcc agggctcgag
      181 catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga
      241 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
      301 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
      361 catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat
      421 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
      481 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
      541 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
      601 aacactcggc tgggaggcct ccaccgagat gctgtacccc gctgacggcg gcctggaagg
      661 cagaagcgac atggcc
//
