LOCUS       sequence-93103-e       10080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector that expresses catalytically inactive form of 
            Flag-tagged human USP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G
  TITLE     Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation 
            of the INK4a tumour suppressor.
  JOURNAL   EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub
            2010 Jul 2.
  PUBMED    20601937
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.129"; journalName: "EMBO J"; date: "2010-08-4- 
            4"; volume: "29"; issue: "15"; pages: "2553-65"
COMMENT     Sequence Label: pQFlag-USP11 CS puroR
FEATURES             Location/Qualifiers
     source          1..10080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2265..2288
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(5208..5781,5782..5784,5785..5793)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5375..5392)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5602..5621
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5813..6412
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5813..5832)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6309..6329
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6786..7211
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7314..7336)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7472..7492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7477..7806
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7657..7792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7828..7847
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7928..7945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8099..8687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8179..8198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8858..9649,9650..9718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9481..9500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9719..9823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9891..9909
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10009..10080,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attggattac aaggatgacg
     2281 atgacaagac cggtatggca gtagccccgc gactgtttgg ggggctctgc ttccgtttcc
     2341 gggaccagaa tccggaagtg gctgttgagg ggcgtcttcc aatctcgcac agctgcgttg
     2401 gctgtagaag agaacggacg gcgatggcga cggtcgcagc aaatccagct gctgctgcgg
     2461 cggctgtggc ggcggcagcg gcggtgactg aggatagaga gccacagcac gaggagctgc
     2521 caggcctgga cagccagtgg cgccagatag aaaacggcga gagtgggcga gaacgtccac
     2581 tgcgggccgg cgaaagctgg ttccttgtgg agaagcactg gtataagcag tgggaggcat
     2641 acgtgcaggg aggggaccag gactccagca ccttccctgg ctgcatcaac aatgccacac
     2701 tctttcaaga tgagataaac tggcgcctca aggagggact ggtggaaggc gaggattatg
     2761 tgctgctccc agcagctgct tggcattacc tggtcagctg gtatggtcta gagcatggcc
     2821 agccacccat tgaacgcaag gtcatagagc tgcccaacat ccagaaggtc gaagtgtacc
     2881 cagtagaact gctgcttgtc cggcacaatg atttgggcaa atctcacact gttcagttca
     2941 gccataccga ttctattggc ctagtattgc gcacagctcg ggagcggttt ctggtggagc
     3001 cccaggaaga cactcggctt tgggccaaga actcagaagg ctctttggat aggttgtatg
     3061 acacacacat cacggttctc gatgcggccc ttgagactgg gcagttgatc atcatggaga
     3121 cccgcaagaa agatggcact tggcccagcg cacagctgca tgtcatgaac aacaacatgt
     3181 cggaagagga tgaggacttc aagggtcagc caggcatctg tggcctcacc aatctgggca
     3241 acacgtcctt catgaactcg gccctgcagt gcctcagcaa tgtgccacag ctcaccgagt
     3301 acttcctcaa caactgctac ctggaggagc tcaacttccg caacccactg ggcatgaagg
     3361 gtgagatcgc agaggcctat gcagacctgg tgaagcaggc gtggtctggc caccaccgct
     3421 ccattgtgcc acatgtgttc aagaacaagg ttggccattt tgcatcccaa tttctgggct
     3481 accagcagca tgactctcag gagctgctgt cattcctcct ggacgggctg catgaggacc
     3541 ttaatcgggt gaagaagaag gagtatgtgg agctgtgcga tgctgctggg cgaccggatc
     3601 aggaggtggc acaggaggca tggcaaaacc acaaacggcg gaacgattct gtgatcgtgg
     3661 acactttcca cggcctcttc aagtccacgc tggtgtgccc cgattgtggc aatgtatctg
     3721 tgaccttcga ccccttctgc tacctcagtg ttccactgcc tatcagccac aagagggtct
     3781 tggaggtctt ctttatcccc atggatccgc gccgcaagcc agagcagcac cggctcgtgg
     3841 tccccaagaa aggcaagatc tcggatctat gtgtggctct gtccaaacac acgggcatct
     3901 cgccagagag gatgatggtg gctgatgtct tcagtcaccg cttctataag ctctatcagc
     3961 tagaggagcc tctgagcagc atcttggacc gtgatgatat cttcgtctat gaggtgtcag
     4021 gtcgcattga ggccattgag ggctcaagag aggacatcgt ggttcctgtc tacctgcggg
     4081 agcgcacccc tgcccgtgac tacaacaact cctactacgg cctgatgctt tttggacacc
     4141 ccctcctggt atcagtgccc cgggaccgct tcacctggga gggcctgtat aacgtcctga
     4201 tgtaccggct ctcacgctac gtgaccaaac ccaactcaga tgatgaggac gatggggatg
     4261 agaaagaaga tgacgaggag gataaagatg acgtccctgg gccctcaact gggggcagcc
     4321 tccgagaccc tgagccagag caggctgggc ccagctctgg agtcacgaac aggtgcccgt
     4381 tcctcctgga caattgcctt ggcacatctc agtggccccc aaggcgacga cgcaagcagc
     4441 tgttcaccct gcagacggtg aactccaatg ggaccagcga ccgcacaacc tcccctgaag
     4501 aagtccatgc ccagccgtac attgctatcg actgggagcc agagatgaag aagcgttact
     4561 atgacgaggt agaggctgag ggctacgtga agcatgactg cgtcgggtac gtgatgaaga
     4621 aggctcccgt gcggctgcag gagtgcattg agctcttcac cactgtggag accctggaga
     4681 aggaaaaccc ctggtactgc ccttcctgca agcagcacca gctggcaacc aagaagctgg
     4741 acctgtggat gctgccggag attctcatca tccacctgaa acgcttttcc tacaccaagt
     4801 tctcccgaga gaagctggac accctcgtgg agtttcctat ccgggacctg gacttctctg
     4861 agtttgtcat ccagccacag aatgagtcga atccggagct gtacaaatat gacctcatcg
     4921 cggtttccaa ccattatggg ggcatgcgtg atggacacta cacaacattt gcctgcaaca
     4981 aggacagcgg ccagtggcac tactttgatg acaacagcgt ctcccctgtc aatgagaatc
     5041 agatcgagtc caaggcagcc tatgtcctct tctaccaacg ccaggacgtg gcgcgacgcc
     5101 tgctgtcccc ggccggctca tctggcgccc cagcctcccc tgcctgcagc tccccaccca
     5161 gctctgagtt catggatgtt aattgactcg agggatccgg aattccgccc ctctccctcc
     5221 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5281 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5341 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5401 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5461 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     5521 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     5581 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5641 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     5701 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5761 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     5821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     5881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     5941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6001 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6361 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6421 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catggctccg accgaagccg
     6481 acccgggcgg ccccgccgac cccgcacccg cccccgaggc ccaccgactc tagtcgaggg
     6541 ctgcagcgct gcagaggccg agtgcagaac tgctccaaag ggacctcaag gctttccgag
     6601 ggacactagg ctgactccat cgagccagtg tagagataag cttatcgatt agtccaattt
     6661 gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
     6721 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
     6781 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     6841 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     6901 ggaacagggt cgaccctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     6961 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     7021 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     7081 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     7141 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     7201 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
     7261 accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     7321 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7381 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     7441 cgtagcgata gcggagtgta gatccggctg tggaatgtgt gtcagttagg gtgtggaaag
     7501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7561 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7801 tgcaaaaagc ttactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7861 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     7921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8521 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     9181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     9481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9841 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9901 tcacgaggcc ctttcgtctt caagaattag cttggccatt gcatacgttg tatccatatc
     9961 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
    10021 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
//
