LOCUS       sequence-93111-e       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector that expresses catalytically inactive form of 
            HA-tagged human USP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G
  TITLE     Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation 
            of the INK4a tumour suppressor.
  JOURNAL   EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub
            2010 Jul 2.
  PUBMED    20601937
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.129"; journalName: "EMBO J"; date: "2010-08-4- 
            4"; volume: "29"; issue: "15"; pages: "2553-65"
COMMENT     Sequence Label: pQHA-USP11 CS puroR
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(5192..5765,5766..5768,5769..5777)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5359..5376)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5586..5605
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5797..6396
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5797..5816)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6293..6313
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6770..7195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7298..7320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7456..7476)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7461..7790
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7641..7776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7812..7831
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7912..7929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8083..8671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8163..8182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8842..9633,9634..9702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9465..9484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9703..9807)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9875..9893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9993..10064,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attgtacgac gtaccagatt
     2281 acgctaccgg tatggcagta gccccgcgac tgtttggggg gctctgcttc cgtttccggg
     2341 accagaatcc ggaagtggct gttgaggggc gtcttccaat ctcgcacagc tgcgttggct
     2401 gtagaagaga acggacggcg atggcgacgg tcgcagcaaa tccagctgct gctgcggcgg
     2461 ctgtggcggc ggcagcggcg gtgactgagg atagagagcc acagcacgag gagctgccag
     2521 gcctggacag ccagtggcgc cagatagaaa acggcgagag tgggcgagaa cgtccactgc
     2581 gggccggcga aagctggttc cttgtggaga agcactggta taagcagtgg gaggcatacg
     2641 tgcagggagg ggaccaggac tccagcacct tccctggctg catcaacaat gccacactct
     2701 ttcaagatga gataaactgg cgcctcaagg agggactggt ggaaggcgag gattatgtgc
     2761 tgctcccagc agctgcttgg cattacctgg tcagctggta tggtctagag catggccagc
     2821 cacccattga acgcaaggtc atagagctgc ccaacatcca gaaggtcgaa gtgtacccag
     2881 tagaactgct gcttgtccgg cacaatgatt tgggcaaatc tcacactgtt cagttcagcc
     2941 ataccgattc tattggccta gtattgcgca cagctcggga gcggtttctg gtggagcccc
     3001 aggaagacac tcggctttgg gccaagaact cagaaggctc tttggatagg ttgtatgaca
     3061 cacacatcac ggttctcgat gcggcccttg agactgggca gttgatcatc atggagaccc
     3121 gcaagaaaga tggcacttgg cccagcgcac agctgcatgt catgaacaac aacatgtcgg
     3181 aagaggatga ggacttcaag ggtcagccag gcatctgtgg cctcaccaat ctgggcaaca
     3241 cgtccttcat gaactcggcc ctgcagtgcc tcagcaatgt gccacagctc accgagtact
     3301 tcctcaacaa ctgctacctg gaggagctca acttccgcaa cccactgggc atgaagggtg
     3361 agatcgcaga ggcctatgca gacctggtga agcaggcgtg gtctggccac caccgctcca
     3421 ttgtgccaca tgtgttcaag aacaaggttg gccattttgc atcccaattt ctgggctacc
     3481 agcagcatga ctctcaggag ctgctgtcat tcctcctgga cgggctgcat gaggacctta
     3541 atcgggtgaa gaagaaggag tatgtggagc tgtgcgatgc tgctgggcga ccggatcagg
     3601 aggtggcaca ggaggcatgg caaaaccaca aacggcggaa cgattctgtg atcgtggaca
     3661 ctttccacgg cctcttcaag tccacgctgg tgtgccccga ttgtggcaat gtatctgtga
     3721 ccttcgaccc cttctgctac ctcagtgttc cactgcctat cagccacaag agggtcttgg
     3781 aggtcttctt tatccccatg gatccgcgcc gcaagccaga gcagcaccgg ctcgtggtcc
     3841 ccaagaaagg caagatctcg gatctatgtg tggctctgtc caaacacacg ggcatctcgc
     3901 cagagaggat gatggtggct gatgtcttca gtcaccgctt ctataagctc tatcagctag
     3961 aggagcctct gagcagcatc ttggaccgtg atgatatctt cgtctatgag gtgtcaggtc
     4021 gcattgaggc cattgagggc tcaagagagg acatcgtggt tcctgtctac ctgcgggagc
     4081 gcacccctgc ccgtgactac aacaactcct actacggcct gatgcttttt ggacaccccc
     4141 tcctggtatc agtgccccgg gaccgcttca cctgggaggg cctgtataac gtcctgatgt
     4201 accggctctc acgctacgtg accaaaccca actcagatga tgaggacgat ggggatgaga
     4261 aagaagatga cgaggaggat aaagatgacg tccctgggcc ctcaactggg ggcagcctcc
     4321 gagaccctga gccagagcag gctgggccca gctctggagt cacgaacagg tgcccgttcc
     4381 tcctggacaa ttgccttggc acatctcagt ggcccccaag gcgacgacgc aagcagctgt
     4441 tcaccctgca gacggtgaac tccaatggga ccagcgaccg cacaacctcc cctgaagaag
     4501 tccatgccca gccgtacatt gctatcgact gggagccaga gatgaagaag cgttactatg
     4561 acgaggtaga ggctgagggc tacgtgaagc atgactgcgt cgggtacgtg atgaagaagg
     4621 ctcccgtgcg gctgcaggag tgcattgagc tcttcaccac tgtggagacc ctggagaagg
     4681 aaaacccctg gtactgccct tcctgcaagc agcaccagct ggcaaccaag aagctggacc
     4741 tgtggatgct gccggagatt ctcatcatcc acctgaaacg cttttcctac accaagttct
     4801 cccgagagaa gctggacacc ctcgtggagt ttcctatccg ggacctggac ttctctgagt
     4861 ttgtcatcca gccacagaat gagtcgaatc cggagctgta caaatatgac ctcatcgcgg
     4921 tttccaacca ttatgggggc atgcgtgatg gacactacac aacatttgcc tgcaacaagg
     4981 acagcggcca gtggcactac tttgatgaca acagcgtctc ccctgtcaat gagaatcaga
     5041 tcgagtccaa ggcagcctat gtcctcttct accaacgcca ggacgtggcg cgacgcctgc
     5101 tgtccccggc cggctcatct ggcgccccag cctcccctgc ctgcagctcc ccacccagct
     5161 ctgagttcat ggatgttaat tgagaattcc gcccctctcc ctcccccccc cctaacgtta
     5221 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5281 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5341 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5401 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5461 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5521 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     5581 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     5641 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5701 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     5761 ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg
     5821 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     5881 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     5941 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6001 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6061 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6121 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6181 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6241 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6301 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc
     6361 gcgacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     6421 ccgaccgaaa ggagcgcacg accccatggc tccgaccgaa gccgacccgg gcggccccgc
     6481 cgaccccgca cccgcccccg aggcccaccg actctagtcg agggctgcag cgctgcagag
     6541 gccgagtgca gaactgctcc aaagggacct caaggctttc cgagggacac taggctgact
     6601 ccatcgagcc agtgtagaga taagcttatc gattagtcca atttgttaaa gacaggatat
     6661 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
     6721 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     6781 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     6841 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc
     6901 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     6961 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     7021 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc
     7081 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     7141 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg
     7201 gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag
     7261 ctggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     7321 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     7381 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     7441 tgtagatccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7501 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7561 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7621 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7681 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7741 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttactg
     7801 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     7861 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     7921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9121 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     9181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     9481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9841 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9901 tcttcaagaa ttagcttggc cattgcatac gttgtatcca tatcataata tgtacattta
     9961 tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata
    10021 gtaatcaatt acggggtcat tagttcatag cccatatatg gagt
//
