LOCUS       sequence-93263-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for genomic targeting of a Tetracycline-inducible Cas9 
            cassette to the human AAVS1/PPP1R12C locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: Puro-Cas9 donor
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    207..231
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(257..304)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagagata tcgtcgacaa gcttatcgat gcggccgcgc tagcacgcgt
      181 cagctgacta gaggatcccc ggatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      241 cacccttgaa ttcttacttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      301 ttttgtcgcc tcccagctga gacaggtcga tccgtgtctc gtacaggccg gtgatgctct
      361 ggtggatcag ggtggcgtcc agcacctctt tggtgctggt gtacctcttc cggtcgatgg
      421 tggtgtcaaa gtacttgaag gcggcagggg ctcccagatt ggtcagggta aacaggtgga
      481 tgatattctc ggcctgctct ctgatgggct tatcccggtg cttgttgtag gcggacagca
      541 ctttgtccag attagcgtcg gccaggatca ctctcttgga gaactcgctg atctgctcga
      601 tgatctcgtc caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct
      661 cgggggagcc cttcagcttc tcatagtggc tggccaggta caggaagttc acatatttgg
      721 agggcagggc cagttcgttt cccttctgca gttcgccggc agaggccagc attctcttcc
      781 ggccgttttc cagctcgaac agggagtact taggcagctt gatgatcagg tcctttttca
      841 cttctttgta gcccttggct tccagaaagt cgatgggatt cttctcgaag ctgcttcttt
      901 ccatgatggt gatccccagc agctctttca cactcttcag tttcttggac ttgccctttt
      961 ccactttggc caccaccagc acagaatagg ccacggtggg
//
