LOCUS       sequence-93394-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP using a self-replicating Venezuelan equine 
            encephalitis (VEE) virus RNA replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-GFP
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcgagctct
       61 aatacgactc actatagatg ggcggcgcat gagagaagcc cagaccaatt acctacccaa
      121 aatggagaaa gttcacgttg acatcgagga agacagccca ttcctcagag ctttgcagcg
      181 gagcttcccg cagtttgagg tagaagccaa gcaggtcact gataatgacc atgctaatgc
      241 cagagcgttt tcgcatctgg cttcaaaact gatcgaaacg gaggtggacc catccgacac
      301 gatccttgac attggaagtg cgcccgcccg cagaatgtat tctaagcaca agtatcattg
      361 tatctgtccg atgagatgtg cggaagatcc ggacagattg tataagtatg caactaagct
      421 gaagaaaaac tgtaaggaaa taactgataa ggaattggac aagaaaatga aggagctggc
      481 cgccgtcatg agcgaccctg acctggaaac tgagactatg tgcctccacg acgacgagtc
      541 gtgtcgctac gaagggcaag tcgctgttta ccaggatgta tacgcggttg acggaccgac
      601 aagtctctat caccaagcca ataagggagt tagagtcgcc tactggatag gctttgacac
      661 cacccctttt atgtttaaga acttggctgg agcatatcca tcatactcta ccaactgggc
      721 cgacgaaacc gtgttaacgg ctcgtaacat aggcctatgc agctctgacg ttatggagcg
      781 gtcacgtaga gggatgtcca ttcttagaaa gaagtatttg aaaccatcca acaatgttct
      841 attctctgtt ggctcgacca tctac
//
