LOCUS       sequence-93461-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 600, Emission:
            650.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Lin MZ, McKeown MR, Ng HL, Aguilera TA, Shaner NC, Campbell RE, 
            Adams SR, Gross LA, Ma W, Alber T, Tsien RY
  TITLE     Autofluorescent proteins with excitation in the optical window for 
            intravital imaging in mammals.
  JOURNAL   Chem Biol. 2009 Nov 25;16(11):1169-79. doi: 
            10.1016/j.chembiol.2009.10.009.
  PUBMED    19942140
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2009.10"; journalName: "Chem Biol"; date: 
            "2009-11-25- 25"; volume: "16"; issue: "11"; pages: "1169-79"
COMMENT     Sequence Label: mNeptune-pBAD
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(128..136,137..151)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagcatgg tgagcaaggg
      181 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
      241 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccgg
      301 cagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctacctg
      361 cttcatgtac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg atttctttaa
      421 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
      481 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
      541 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
      601 ggccagcacc gagaccctgt accccgctga cggcggcctg gaaggcagat gcgacatggc
      661 cctgaagctc gtgggcgggg gccacctgat ctgcaacctg aagaccacat acagatccaa
      721 gaaacccgct aagaacctca agatgcccgg cgtctacttt gtggaccgca gactggaaag
      781 aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata
      841 ctgcgacctc cctagc
//
