LOCUS       sequence-93463-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 548, Emission:
            561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: mKO-pBAD
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagtgtgat taaaccagag atgaagatga ggtactacat ggacggctcc
      241 gtcaatgggc atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat
      301 caagagatga cactacgcgt cacaatggcc aagggcgggc caatgccttt cgcgtttgac
      361 ttagtgtcac acgtgttctg ttacggccac agacctttta ctaaatatcc agaagagata
      421 ccagactatt tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc
      481 gaagatggtg ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac
      541 cacaaatcca aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa
      601 agtgttgatt gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt
      661 gatgttacga tgtacctaaa acttgaagga ggcggcaatc acaaatgcca attcaagact
      721 acttacaagg cggca
//
