LOCUS       sequence-9347-ex        3273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Honda A, Adams SR, Sawyer CL, Lev-Ram V, Tsien RY, Dostmann WR
  TITLE     Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate 
            revealed by a genetically encoded, fluorescent indicator.
  JOURNAL   Proc Natl Acad Sci U S A. 2001 Feb 27. 98(5):2437-42.
  PUBMED    11226257
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2001 Feb 27. 98(5):2437-42."
COMMENT     Sequence Label: pcDNA3.1(-)-Cygnet2.1
FEATURES             Location/Qualifiers
     source          1..3273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(61..63,64..66,67..579)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(2536..2538,2539..2541,2542..3252)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 caaggcaatg cgtttaacgg gccctctaga ctcgagcagc tgaagcttgc ggccgccacc
       61 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      121 ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      241 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      301 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc
      361 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      421 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      481 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
      541 ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      601 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      661 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      721 ctgctggagt tcgtgaccgc cgcccgcatg caggcattcc ggaagttcac caaatccgaa
      781 aggtccaagg atctcataaa ggaggccatc cttgacaatg actttatgaa gaacttggag
      841 ctgtcacaga tccaagagat tgtggattgt atgtacccag tggagtacgg caaagacagc
      901 tgcatcatca aagaaggaga tgtggggtca ctggtgtatg tcatggaaga tggtaaggtt
      961 gaagttacaa aagaaggcgt gaagctgtgc acaatgggtc ctggtaaagt gtttggagag
     1021 ttggctatcc tttacaactg tacccggacg gcgaccgtca aaactcttgt aaatgtgaaa
     1081 ctctgggcca ttgatcgaca atgttttcag acgataatga tgaggacagg acttatcaag
     1141 cataccgagt atatggaatt tttaaaaagc gttccaacat tccagagcct tcctgaagag
     1201 atcctcagta agcttgctga cgtccttgaa gagacccact atgaaaatgg ggaatatatc
     1261 atcaggcaag gtgcaagagg ggacaccttc tttatcatca gtaaaggaaa ggttaatgtc
     1321 actcgtgaag actcgcccaa tgaagaccca gtctttctta gaaccttagg aaaaggagat
     1381 tggtttggag agaaagcctt gcagggggaa gatgtgagaa cagcgaatgt aattgcggca
     1441 gaagctgtaa cctgccttgt gatcgacaga gactctttca aacatttgat tggaggatta
     1501 gatgatgttt ctaataaagc atatgaagat gcagaagcta aggcaaaata tgaagctgaa
     1561 gctgctttct tcgccaacct gaagctgtct gatttcaaca tcattgacac ccttggagtt
     1621 ggaggtttcg gacgagtaga actggtccag ttaaaaagtg aagaatccaa aacctttgca
     1681 atgaagattc tcaagaaacg ccacatcgtg gatacaagac agcaggaaca catccgctcg
     1741 gagaagcaga tcatgcaggg ggcccattcg gacttcatag tgagattata cagaacattt
     1801 aaggacagca aatatttgta tatgttgatg gaagcttgcc taggtggaga gctctggacc
     1861 attctcaggg atcgggggtc atttgaagat tctacaacca gattttatac agcatgtgtg
     1921 gtagaagctt ttgcctatct gcattccaaa ggaatcattt acagggacct caagcctgaa
     1981 aatctcatcc tagatcaccg aggttatgcc aaactggttg attttggctt tgcaaagaaa
     2041 ataggatttg gaaagaaaac atgggctttt tgtgggactc cagaatatgt agccccagag
     2101 atcatcctga acaaaggcca tgacatttca gccgactatt ggtcactggg aatcctcatg
     2161 tatgagcttc tgactggcag cccacctttc tcaggcccag atcctatgaa aacctataac
     2221 atcatattga gggggattga catgatagag tttccaaaga agattgccaa aaatgctgct
     2281 aatttaatta aaaaactatg cagggataat ccatcagaaa gattagggaa tttgaaaaac
     2341 ggagtgaaag acattcaaaa gcacaaatgg tttgagggct ttaattggga aggcttaaga
     2401 aaaggcacct tgacacctcc tataatacca agtgttgcat cacccacaga cacaagcaat
     2461 tttgacagtt tccctgagga caatgacgaa ccgccacctg atgacaactc aggatgggac
     2521 atagacttcg agctcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2581 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2641 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2701 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tgatgtgctt cgcccgctac
     2761 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2821 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2881 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2941 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3001 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3061 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3121 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     3181 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3241 gagctgtaca agtaagaatt catcgatgat atc
//
