LOCUS       sequence-93473-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Qin Y, Miranda JG, Stoddard CI, Dean KM, Galati DF, Palmer AE
  TITLE     Direct comparison of a genetically encoded sensor and small molecule
            indicator: implications for quantification of cytosolic Zn(2+).
  JOURNAL   ACS Chem Biol. 2013 Nov 15;8(11):2366-71. doi: 10.1021/cb4003859. 
            Epub 2013 Sep 3.
  PUBMED    23992616
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb4003859";
            journalName: "ACS Chem Biol"; date: "2013-11-15- 15"; volume: "8"; 
            issue: "11"; pages: "2366-71"
COMMENT     Sequence Label: pcDNA-NES-ZapCY2
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..111
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(388..409)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     737..756
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
       61 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      121 caccccggtg aacagctcct cgcccttgct caccatgagc tcgataccat gttgacaatt
      181 aatatggttc actatggaac atgtatcatc tccaaggaaa tcacagtcct cccaattatg
      241 agctaatggt tccatagggt gtttgaaatc ttgagagaca tgatccttca aaagatgtct
      301 ttgtaggtca aatagtgagc tacaagactc aggacattct ttccatttgt gttttaagtc
      361 attgttttta tgcatgcggg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      421 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
      481 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      541 gtcctcgatg ttgtggcgga tcttgaagtg ggccttgatg ccgttcttct gcttgtcggc
      601 ggtgatatag acgttgtggc tgatgtagtt gtactccagc ttgtgcccca ggatgttgcc
      661 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      721 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tacgctcctg
      781 gacgta
//
