LOCUS       sequence-93509-e        1219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1219)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X1
FEATURES             Location/Qualifiers
     source          1..1219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     351..370
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        351..369
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    464..488
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1106..1205)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cggtcacggc caggttagtc gccatttttc attcgtacat catggcacgt acttggcatg
       61 tttcagaaac antngcgcat cgggcctgcc attcaaggcg atagatgtcg gcacctgatt
      121 ggccgacata ttcggcgagc cccaattata ccaatataaa tcagcatcca tgttggaatt
      181 aatcgcggcc tcgacgtttc ccgtggaata tggctcataa acacccttgt attactgttt
      241 atgtaagcag acagtttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      301 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
      361 cactataggg gatatcagcc tggatggcaa ataatgattt tatttttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaagaa
      481 agctgggttg ctccttccat ctcttgtctt tgctctcacc tctctctaag gcatcatgag
      541 tctacagtgt agctccaggt ccctgtgccg cggtggcggt ggcagcagga acttcagctc
      601 aggctctgct ggcctagtca gcttccagcg cagatccacc agcagctcta tgcgccgcag
      661 cggtggaggt ggtggtggga gattttcagg aggaggcttc tgtgggagtt ctggtagtgg
      721 ctttggaagt aagagtctta tgaaccttgg aggcggtagg agcatctcca agagtgtggc
      781 tggaggtggt ggcagcttct gtggtggctt tgggggtggc agctacggcg gtggtggctt
      841 tgggggtggc agctacggcg gtggtggctt tgggggtggc agcttcggcg gtggtggctt
      901 tggagggagt ggttttggcg gaggtcttgg tggtggtggt ggttttggta gtggaggtgg
      961 ttttggtggt ggtagatttg gtagtatggg gcctgtctgt ccccctggtg gcatccagga
     1021 agtgaccatc aaccaaagcc ttctgcaacc cctcaacgtt gaggttgacc ctcagattca
     1081 aaaagtgaag tctcaggaga gagaaagcct gcttttttgt acaaagttgg cattataaaa
     1141 aagcattgct catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcata
     1201 ttgcatttgt agtccaccg
//
