LOCUS       sequence-93575-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector expressing a fusion protein of Lamp1, 
            mRFP, and a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM
  TITLE     mTORC1 senses lysosomal amino acids through an inside-out mechanism 
            that requires the vacuolar H-ATPase.
  JOURNAL   Science. 2011 Nov 4;334(6056):678-83.
  PUBMED    22053050
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-11-4"; volume: "334(6056)"; pages: "678-83"
COMMENT     Sequence Label: LAMP1-mRFP-FLAG
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    130..247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(292..339)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     primer_bind     complement(399..422)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(597..616)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg gctgccttgg
       61 aaaaggcgca accccaaccc cctcgagccg cggccaaagt ggatctctgc tgtccctgta
      121 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
      181 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
      241 tttaaaattg tggatgaata ctgccatttg tctcgaggtc gagaattctc acttgtcgtc
      301 atcgtctttg tagtccttgt cgtcatcgtc tttgtagtcg gcgccggtgg agtggcggcc
      361 ctcggcgcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      421 cttgatgtcg gtcttgtagg cgccgggcag ctgcacgggc ttcttggcca tgtaggtggt
      481 cttgacctcg gcgtcgtagt ggccgccgtc cttcagcttc agcctcatct tgatctcgcc
      541 cttcagggcg ccgtcctcgg ggtacatccg ctcggtggag gcctcccagc ccatggtctt
      601 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      661 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc c
//
