LOCUS       sequence-93609-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-OKS-iG
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      642..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttcattgttg tcagcttcct ccacccactt ctgcagcaag ggccgcagct tacacatgtt
       61 cttgaagcta agctgcagag cctcaaagcg gcagatggtc gtttggctga ataccttccc
      121 aaatagaacc cccagggtga gccccacatc ggcctgtgta tatcccaggg tgatcctctt
      181 ctgcttcagg agcttggcaa attgctcgag ttctttctgc agagctttga tgtcctggga
      241 ctcctccggg ttttgctcca gcttctcctt ctccagcttc acggcaccag gggtgacggt
      301 gcagggctcc ggggaggccc catcggagtt gctctccacc ccgactcctg cttcgccctc
      361 aggctgagag gtctccaagc cgccttgggg cactagcccc actccaacct ggggcccaca
      421 gtacgccatc cccccacaga actcatacgg cggggggcat gggggaatcc cccacacctc
      481 agagcctggc ccaacccccg gcccgattcc tggccctcca ggagggcctt ggaagcttag
      541 ccaggtccga ggatcaaccc agcccggctc cggcccccct ggcccatcac ctccaccacc
      601 tggagggggc gagaaggcga aatccgaagc caggtgtccc gccatggtgg cgaattcacg
      661 cgcccatatg ctagacttgg cggactagac tatgtcgtag tccattcagg ttagccgtag
      721 agagttatag gggcccctct caggtagctg aatgatttaa cactgctagc tagagtagtc
      781 atggcca
//
