LOCUS       sequence-93611-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP using a self-replicating Venezuelan equine 
            encephalitis (VEE) virus RNA replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-GFP
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgctcacca tggtggcgaa ttgaatcgat gctgagggcg cgcccatatg ctagacttgg
       61 cggactagac tatgtcgtag tccattcagg ttagccgtag agagttatag gggcccctct
      121 caggtagctg aatgatttaa cactgctagc tagagtagtc atggccataa ctatgatgga
      181 agttcctacg gtttcatacc ttgattctac tgccttgcac agctctgaaa gaatacccac
      241 tcggttccag cgtgttgact cttcatgcaa tgcccttctc ctgtcatcat catgttcatc
      301 gtctgctgcc agaggtttgc caagcttaaa cagccttttt agggggtctg ccacacggca
      361 cgctgtgccg gtcacggagt cacacaaaat aaaccctcca cagaaataag gcgctttctc
      421 gcccaccaca gcatctataa tcttgacttc catattcaac caggtggcgc acctgtctgc
      481 cattaatttg tccgatttga ctcctttcac gatattgtca tctccaatga atgctgcaca
      541 tggtgatccg gttagccgtt ctctcaacac tctgcttgcg attacaatgt taatgactgt
      601 gttcacaaac agtgtgagga acattccaga tttcatcatg gctccgaatt taaatttagt
      661 tttagtgggc aaatgtattg atgaaatttc gccgaaagcc gcctcaatca gcgtcaacag
      721 ctctgcgtcc acacctaagt cttccagaat cattaacgcg gtcagagcca tggcgtcgtc
      781 ctcac
//
