LOCUS       sequence-93645-e        3820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSALECT with AhyI insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-AhyI
FEATURES             Location/Qualifiers
     source          1..3820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             770..1558
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(916..935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1862..2521)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2436..2455
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2522..2624)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2844..3432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3333..3352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3586..3603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3720..3741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3756..3761,3762..3779,3780..3786)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggatctcgac atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
       61 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgctaacgc cgcgacgtgc
      121 gactgcgcat atggaattca tgttggtatt caaaggtaag ttgaaagagc acccacgttg
      181 ggaagttgaa aacgaattat acaggtttag aaaccgtgta ttctcagata gattgggatg
      241 ggatgttgag agtcacaggg gtctagagca ggactctttc gacaccccag atacgcattg
      301 ggtgttaatc gaagacgagg aagggttgtg tggttgcatc cgtcttctta gttgtgcgaa
      361 agattacatg ctgccaagta tattccccac cgctcttgca ggtgaggcgc ctcccagaag
      421 caacgatgtc tgggagctga ccaggctagc catagatgcg gagagggctc cgagactagg
      481 caacggtatc tcagaactaa catgcatcat ctttagggaa gtctacgcat tcgcaaaagc
      541 gcagggcatc agggaactgg ttgcggtggt ttcactgccg gttgaaagga tttttcgtag
      601 gttgggccta cctatcgagc gtcttggtca ccgtcaagca gtggatttag gtgccgttag
      661 aggagtgggg atcaggttcc atcttgacga acgtttcgca cgtgcagtag gtcagccctt
      721 gcaaggtgca tacgacgagg cacgtgagct tgtaaccgaa ggatcccacc cagaaacgct
      781 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      841 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      901 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
      961 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1021 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1081 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1141 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1201 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     1261 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1321 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1381 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1441 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1501 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaaga
     1561 tttttccaat gatgagcact ttttgatcag aaaaaaagga tcatatcgtc aattattacc
     1621 tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg
     1681 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     1741 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     1801 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
     1861 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1921 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1981 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2041 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2101 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2161 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2221 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2281 caccgtcttt cattgccata cgtaattccg gatgagcatt catcaggcgg gcaagaatgt
     2341 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2401 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2461 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2521 ttttagcttc cttagctcct gaaaatctcg acaactcaaa aaatacgccc ggtagtgatc
     2581 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     2641 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     2701 agtgatcttc cgtcacaggt atttattcgg tcgaaaagga tctaggtgaa gatccttttt
     2761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3481 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3541 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3601 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3661 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3721 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     3781 atgttgtgtg gaattgtgag cgggataaca atttcacaca
//
