LOCUS       sequence-93653-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of Patronin CC+CKK (using Baculodirect 
            kit from Invitrogen).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pENTR-GFP-Patronin CC+CKK
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     148..169
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(224..244)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(265..364)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(380..397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(380..396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(389..411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      460..546
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
       61 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      121 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      181 ccccggtgaa cagctcctcg cccttgctca ccatggtgaa ggagccctga aaatacaggt
      241 tttcctcgag tacggcggcc gcggagcctg cttttttgta caaagttggc attataaaaa
      301 agcattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      361 tttggggccc gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc
      421 atgctagcgt taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
      481 tcggaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      541 acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg gtggcgggca
      601 ggacgcccgc cataaactgc caggcatcaa actaagcaga agg
//
