LOCUS       sequence-93688-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mVenus-fusion protein of VfAU1-LOV domain (Vaucheria 
            frigida Aureochrome 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: mV-VfAU1-LOV_226
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(92..94,95..97,98..610)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(137..158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(398..417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcggcgccg gtaccggtag catggtgagc aagggcgagg agctgttcac
      121 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      181 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
      241 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
      301 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      361 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      421 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      481 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      541 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      601 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      661 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca aactgagcaa
      721 agaccccaac gagaagcgcg atcacatgg
//
