LOCUS       sequence-93694-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: Opto-mFGFR1_204
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..155
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagaca
       61 caagctggct agcgtttaaa cgggccctct agactcgagc ggccgcggcc accatgggga
      121 gtagcaagag caagcctaag gaccccagcc agcgcctcga catgaagagc ggcaccaaga
      181 agagcgactt ccatagccag atggctgtgc acaagctggc caagagcatc cctctgcgca
      241 gacaggtaac agtgtcagct gactccagtg catccatgaa ctctggggtt ctcctggttc
      301 ggccctcacg gctctcctcc agcgggaccc ccatgctggc tggagtctcc gaatatgagc
      361 tccctgagga tccccgctgg gagctgccac gagacagact ggtcttaggc aaaccacttg
      421 gcgagggctg cttcgggcag gtggtgttgg ctgaggccat cgggctggat aaggacaaac
      481 ccaaccgtgt gaccaaagtg gccgtgaaga tgttgaagtc cgacgcaacg gagaaggacc
      541 tgtcggatct gatctcggag atggagatga tgaaaatgat tgggaagcac aagaatatca
      601 tcaaccttct gggagcgtgc acacaggatg gtcctcttta tgtcattgtg gagtacgcct
      661 ccaaaggcaa tctccgggag tatctacagg cccggaggcc tcctgggctg gagtactgct
      721 ataaccccag ccacaacccc gaggaacagc tgtcttccaa agatctggta tcctgtgcct
      781 atcaggtggc tcggggcatg gagtatcttg cctctaagaa gtgtatacac cgagac
//
