LOCUS       sequence-93708-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucC-hDib1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-CO-hDib1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(548..574)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             589..612
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttatgg gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
      121 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
      181 cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc
      241 cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca
      301 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
      361 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
      421 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
      481 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg
      541 ggtaccggtg gtggtggtgg tagtggtggt ggtggatccg ccaccatgga ctacaaggac
      601 gacgatgaca aggaactagc tctagttgaa ttcatgtcgt acatgctccc gcacctgcac
      661 aacggctggc aggtggacca ggccatcctc tcggaggagg accgcgtggt cgtcatccgc
      721 ttcggccacg actgggatcc tacgtgcatg aagatggacg aggtcctgta cagcatcgcc
      781 g
//
