LOCUS       sequence-93822-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger 
            K
  TITLE     A high-efficiency system for the generation and study of human 
            induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
  PUBMED    18786420
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Sep 11. 3(3):340-5."
COMMENT     Sequence Label: FU-tet-o-hc-myc
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             104..130
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             143..169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             149..175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             188..214
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             194..220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattgat
       61 cttcggccag cgactagtag aagccaccat ggatagctat ccttatgacg tgcctgacta
      121 tgccagcctg atgctgatga gctatcctta tgacgtgcct gactatgcca gcctgatgct
      181 gatgagctat ccttatgacg tgcctgacta tgccagcctg atgctgatgt ctagacccct
      241 caacgttagc ttcaccaaca ggaactatga cctcgactac gactcggtgc agccgtattt
      301 ctactgcgac gaggaggaga acttctacca gcagcagcag cagagcgagc tgcagccccc
      361 ggcgcccagc gaggatatct ggaagaaatt cgagctgctg cccgcgccgc ccctgtcccc
      421 tagccgccgc tccgggctct gctcgccctc ctacgttgcg gtcacaccct tctcccttcg
      481 gggagacaac gacggcggtg gcgggagctt ctccacggcc gaccagctgg agatggtgac
      541 cgagctgctg ggaggagaca tggtgaacca gagtttcatc tgcgacccgg acgacgagac
      601 cttcatcaaa aacatcatca tccaggactg tatgtggagc ggcttctcgg ccgccgccaa
      661 gctcgtctca gagaagctgg cctcctacca ggctgcgcgc aaagacagcg gcagcccgaa
      721 ccccgcccgc ggccacagcg tctgctccac ctccagcttg tacctgcagg atctgag
//
