LOCUS       sequence-93867-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 transfer vector, encodes firefly luciferase gene interrupted 
            by a gamma-globin intron. The reporter gene is silent in the 
            transfected cells and expressed in the infected cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Mazurov D, Ilinskaya A, Heidecker G, Lloyd P, Derse D
  TITLE     Quantitative comparison of HTLV-1 and HIV-1 cell-to-cell infection 
            with new replication dependent vectors.
  JOURNAL   PLoS Pathog. 2010 Feb 26;6(2):e1000788. doi: 
            10.1371/journal.ppat.1000788.
  PUBMED    20195464
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2010-02-26"; volume: "6(2):e1000788. doi"; pages: "
            10.1371/journal.ppat.1000788"
COMMENT     Sequence Label: pUCHR-inLuc
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(117..135)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttgtgagcgc tcacaattcc ggtagcgcta ccggactcag atctgccacc atggaagacg
       61 ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag
      121 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      181 atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg
      241 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
      301 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
      361 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta
      421 ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc
      481 caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
      541 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag
      601 agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc
      661 tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag
      721 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
      781 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
      841 taatgtatag a
//
