LOCUS       sequence-93874-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 transfer vector, encodes firefly luciferase gene interrupted 
            by a gamma-globin intron. The reporter gene is silent in the 
            transfected cells and expressed in the infected cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Mazurov D, Ilinskaya A, Heidecker G, Lloyd P, Derse D
  TITLE     Quantitative comparison of HTLV-1 and HIV-1 cell-to-cell infection 
            with new replication dependent vectors.
  JOURNAL   PLoS Pathog. 2010 Feb 26;6(2):e1000788. doi: 
            10.1371/journal.ppat.1000788.
  PUBMED    20195464
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2010-02-26"; volume: "6(2):e1000788. doi"; pages: "
            10.1371/journal.ppat.1000788"
COMMENT     Sequence Label: pUCHR-inLuc
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(230..274)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    459..692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
       61 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
      121 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
      181 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
      241 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
      301 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
      361 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
      421 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
      481 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
      541 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
      601 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
      661 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
      721 ccactcttct ctttgccttg gtgggtgcta ctcctaat
//
