LOCUS       sequence-94019-e         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged EGFP-mOrange fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT
  TITLE     Comprehensive analysis of RNA-protein interactions by 
            high-throughput sequencing-RNA affinity profiling.
  JOURNAL   Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 
            2014 May 8.
  PUBMED    24809628
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2970"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "683-8"
COMMENT     Sequence Label: EGFP-mOrange pHis-//
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..43
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(96..113)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(398..417)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtttagagg ccccaagggg ttatgctagt tattgctcag cggtggcagc agccaactca
       61 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
      121 tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt
      181 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgcc gacgatgtag
      241 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc ggaggtgtag
      301 tggccgccgt ccttcagctt cagcctcatc ttgatctcgc ccttcagggc gccgtcctcg
      361 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      421 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctggagg
      481 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
      541 ttgaagccct cggggaagga cagcttgaa
//
