LOCUS       sequence-94046-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of Mac-GFP under the Syn promoter, in 
            floxed/reversed (Cre-dependent) manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Chow BY, Han X, Dobry AS, Qian X, Chuong AS, Li M, Henninger MA, 
            Belfort GM, Lin Y, Monahan PE, Boyden ES
  TITLE     High-performance genetically targetable optical neural silencing by 
            light-driven proton pumps.
  JOURNAL   Nature. 2010 Jan 7. 463(7277):98-102.
  PUBMED    20054397
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 7. 463(7277):98-102."
COMMENT     Sequence Label: pAAV-Syn-FLEX-Mac-GFP
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     311..332
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(391..424)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(487..520)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(536..983)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
       61 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      121 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      181 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      241 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
      301 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      361 acgagctgta caagtaatga gctagctagt ataacttcgt atagcataca ttatacgaag
      421 ttatgggtcg atggtgatgc ttggcaattc gggtcgatgg tgaagcattg gtcttcctat
      481 tttgatataa cttcgtatag gatactttat acgaagttat gggaattctt ctcgactgcg
      541 ctctcaggca cgacacgact cctccgctgc ccaccgcaga ctgaggcagc gctgagtcgc
      601 cggcgccgca gcgcagatgg tcgcgcccgt gcccccctat ctcgcgcctc gcgtggtgcg
      661 gtccggctgg gccggcggcg gcgcggacgc gaccaaggtg gccgggaagg ggagtttgcg
      721 ggggaccggc gagtgacgtc agcgcgcctt cagtgctgag gcggcggtgg cgcgcgccgc
      781 caggcggggg cgaaggcact gtccgcggtg ctgaagctgg cagtgcgcac gcgcctcgcc
      841 gcatcctgtt tcccctcccc ctctctgata ggggatgcgc aatttgggga atgggggttg
      901 ggtgcttgtc cagtgggtcg gggtcggtcg tcaggtaggc acccccaccc cgcctcatcc
      961 tggtcctaaa acccacttgc actcatacgc agggccctct gcagtctaga cacttaatta
     1021 accatttgct gccgacccct ccccccaact tctcggggac tgtgg
//
